In this websever, we scanned all potential CRISPR/Cas9 targets on zebrafish genome exons (Refseq annotation, with NGG as PAM sequence) and evaluated each targets from the following four aspects:
1. Chromatin Accessibility status labeled by "OC" or "CC"
2. Sequence feature evaluation calculated by SSC
3. Potential off-target site evaluated by sequence similarity with other site of genome
Once an user submitting a query via a gene name (both Refseq ID and official gene symbol are supported), the matching records on NL/OC region and with few potential off-target site will be given high priority to return to users. If no preferential targets returned, all matching result will be returned.
Briefly, the three aspects of evaluation standard were identified as follow:
Chen Y, Zeng S, Hu R, et al. Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish.[J]. Plos One, 2017, 12(8):e0182528.
You can submit a gene name (both Refseq ID and official gene symbol are supported) and exon number (also chromatin accessibility status, optional) to get potential Cas9 cleavage target sites with following information: